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anti jacalin  (Vector Laboratories)


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    Vector Laboratories anti jacalin
    Anti Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+jacalin/Biotinylated+Jacalin/pmc12672989-84-46-48
    Average 94 stars, based on 85 article reviews
    anti jacalin - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility
    Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [anti-PNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.

    Article Title: Epididymal epithelial cells facilitate NEU1 loading to modulate sperm α-2,6 sialylation, enhance maturation and motility.
    Article Snippet: Blocking was conducted with 3% BSA (BIOFROXX) in permeabilization buffer at RT for 1 h with rotation. .. Primary antibodies (anti-NEU1 [1:100, GenScript], anti-NEU3 [1:1000, GenScript], Biotinylated Maackia Amurensis Lectin I [anti-MAL-I, 1:50, Vector Labs], Biotinylated Maackia Amurensis Lectin II [anti-MAL-II, 1:50, Vector Labs], biotinylated Sambucus Nigra Lectin [anti-SNA, 1:50, Vector Labs], biotinylated Wheat Germ Agglutinin [anti-WGA, 1:50, Vector Labs], Peanut Agglutinin [antiPNA, 1:100], anti-Jacalin [1:300, Vector Labs], Alexa Fluor 594 anti-biotinylated Phosphotyrosine [1:200, Biolegend]), anti-EGFR [1:300, ABclonal], anti-Phospho-EGFR [1:100, ABclonal], JC-1 [1:200, Beyotime], DCFH-DA [1:50, Beyotime] were incubated overnight at 4 °C. .. The next day, samples were centrifuged, washed with PBST, and incubated with secondary antibodies (PE Donkey anti-rabbit IgG [1:1000, Biolegend], FITC Streptavidin [1:500, Biolegend], DyLight 488 Streptavidin [1:500, Biolegend]) in permeabilization buffer at RT for 1 h. After PBST washes, samples were stained with DAPI (Beyotime) and analyzed using a CytoFLEX flow cytometer.



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    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by <t>Jacalin</t> lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.
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    (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with <t>jacalin-FITC</t> (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.
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    Image Search Results


    X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.

    Journal: Journal of Cancer

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation

    doi: 10.7150/jca.25252

    Figure Lengend Snippet: X-ray irradiation induces the expression of C1GalT1 and core 1 O-glycans in esophageal cancer cells. (A) C1GalT1 mRNA expression in esophageal cancer cells 48 h after irradiation was measured by qPCR. (B) C1GalT1 protein expression in esophageal cancer cells 48 h after irradiation was measured by western blot. (C) Expression of core 1 O-glycans was detected by flow cytometry. (D) Core 1 O-glycans were analyzed by Jacalin lectin blot. (E) Expression of core 1 O-glycans in esophageal cancer cells 48 h after irradiation was measured by flow cytometry. *P<0.05.

    Article Snippet: The blotted polyvinylidene (PVDF) membranes were incubated with antibodies against C1GalT1 (ab57492; Abcam, 1:1000), GAPDH (AF1186;Beyotime,1:2000), β1-integrin(ab24693; Abcam, 1:1000), Try397 pFAK (sc81493; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:800), Ser 473 pAkt (sc293125; Santa Cruz Biotechnology, 1:800) and biotinylated Jacalin (B1115; Vector Laboratories, Burlingame, CA, 1:1000).

    Techniques: Irradiation, Expressing, Western Blot, Flow Cytometry

    C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.

    Journal: Journal of Cancer

    Article Title: Knockdown of C1GalT1 inhibits radioresistance of human esophageal cancer cells through modifying β1-integrin glycosylation

    doi: 10.7150/jca.25252

    Figure Lengend Snippet: C1GALT1 modifies O-glycans on β1-integrin. (A)Expression of core 1 O-glycans was detected by flow cytometry. (B) Core 1 O-glycans were analyzed by Jacalin lectin blot. (C) Cell lysates were pulled down (PD) by Jacalin and immunoblotted with an anti-β1-integrin antibody. (D) Cell lysates were immunoprecipitated with an anti-β1-integrin antibody and blotted with Jacalin or anti-β1-integrin antibody. **P<0.001.

    Article Snippet: The blotted polyvinylidene (PVDF) membranes were incubated with antibodies against C1GalT1 (ab57492; Abcam, 1:1000), GAPDH (AF1186;Beyotime,1:2000), β1-integrin(ab24693; Abcam, 1:1000), Try397 pFAK (sc81493; Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1:800), Ser 473 pAkt (sc293125; Santa Cruz Biotechnology, 1:800) and biotinylated Jacalin (B1115; Vector Laboratories, Burlingame, CA, 1:1000).

    Techniques: Expressing, Flow Cytometry, Immunoprecipitation

    (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with jacalin-FITC (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.

    Journal: PLoS ONE

    Article Title: Temporal dynamics of ovine airway epithelial cell differentiation at an air-liquid interface

    doi: 10.1371/journal.pone.0181583

    Figure Lengend Snippet: (A) Ovine tracheal epithelial cell cultures were grown at ALI for the indicated number of days (relative to establishment of the ALI), fixed and processed for SEM. (B) Ovine tracheal epithelial cell cultures were grown for the indicated number of days, fixed and stained with jacalin-FITC (green), rhodamine-phalloidin (red) and DAPI (blue). Mucus globules are indicated by white arrows, carpets of amorphous mucus are indicated by white arrowheads and jacalin-labelled mucin-positive cells are indicated by yellow arrows.

    Article Snippet: The apical surfaces were washed three times for 3 min with PBS and blocked with PBST (PBS with 0.1% Tween-20) containing 10% (v/v) normal goat serum and 1% (w/v) BSA for 1 h. The ALI cultures were incubated for 1 h with primary antibodies diluted in blocking buffer to the following concentrations: rabbit anti-β-tubulin antibody (Abcam #ab6046) 1:200, mouse anti-ZO1 antibody (Thermofisher #33–9100) 1:50 and Jacalin-FITC lectin (Vector #FL-1151) 1:25.

    Techniques: Staining